Figure 7.

LPCE and LPF1 modulate the immune system of G. mellonella. (A) LPCE: The group of LPCE + PBS increased the hemocyte number compared to a PBS control (PBS + PBS). The group LPCE + C. auris also increased the hemocyte quantity compared to C. auris group (PBS + C. auris). (B) LPF1: The group of LPF1 + PBS increased the hemocyte number compared to a PBS control (PBS + PBS). The group LPF1 + C. auris also increased the hemocyte quantity compared to C. auris group (PBS + C. auris). PBS + C. auris group showed a reduction of hemocyte quantity in relation to the PBS control group, but when the larvae were pretreated with LPCE or LPF1, the hemocyte quantity was very similar to the values found in the PBS control group. (C,D) LPCE and LPF1 increased the gene expression of galiomicin of G. mellonella. Relative quantification (log) of galiomicin for the groups treated with only PBS (Control), pre-treated with PBS and infected with C. auris, only treated with LPCE or LPF1, and pre-treated with LPCE or LPF1 and infected with C. auris. The units in the Y-axis were calculated based on the 2^−ΔΔCT method, and they are expressed as the means and standard deviation. Galiomicin expression was normalized and compared with the expression of insects exposed to the control (PBS) using the reference gene β-actin. Different letters (A, B, and C) represent statistically significant differences among the groups. ANOVA and Tukey Tests (p < 0.05 was considered significant). ***p ≤ 0.001.