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. 2020 Aug 10;10:13466. doi: 10.1038/s41598-020-70311-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Osmotic preconditioning induces elevation of glycerol biosynthesis. (A–C) 2D-thin layer chromatography of ¹⁴C-acetate labelled metabolites from dauers that were non-preconditioned, chamber and osmotic preconditioned respectively. Enumerated spots indicate trehalose (1), glucose (2), glutamate (3), glutamine (4), and alanine (5). Representative images from at least two independent experiments performed on two different days. (D,E) Chromatogram of glycerol standard and spot add. scrapped out from osmotic preconditioned 2D-TLC. (F,G) Mass spectrum of glycerol standard and spot add. from osmotic preconditioned sample.