Table 2.

Analysis of variance for adsorption of malachite green by E. intestinalis obtained by FCCCD.

Source of variance		Degrees of freedom	Sum of square	Mean of square	F-value	P-value	Coefficient estimate
Overall model		20	43,277.76	2,163.89	127.64	< 0.0001*	77.10
Linear effect	X1	1	29,967.77	29,967.77	1767.73	< 0.0001*	29.69
	X2	1	336.13	336.13	19.83	0.0001*	3.14
	X3	1	4.90	4.90	0.29	0.5948	− 0.38
	X4	1	450.05	450.05	26.55	< 0.0001*	3.64
	X5	1	970.12	970.12	57.23	< 0.0001*	− 5.34
Interaction effect	X1X2	1	376.50	376.50	22.21	< 0.0001*	− 3.43
	X1X3	1	195.72	195.72	11.55	0.0020*	2.47
	X1X4	1	74.46	74.46	4.39	0.0449*	− 1.53
	X1X5	1	421.37	421.37	24.86	< 0.0001*	3.63
	X2X3	1	14.51	14.51	0.86	0.3625	− 0.67
	X2X4	1	335.70	335.70	19.80	0.0001*	3.24
	X2X5	1	161.12	161.12	9.50	0.0045*	− 2.24
	X3X4	1	2.35	2.35	0.14	0.7125	0.27
	X3X5	1	401.18	401.18	23.66	< 0.0001*	3.54
	X4X5	1	41.52	41.52	2.45	0.1284	1.14
Square effect	X12	1	3,420.41	3,420.41	201.76	< 0.0001*	− 37.19
	X22	1	4.30	4.30	0.25	0.6184	1.32
	X32	1	860.06	860.06	50.73	< 0.0001*	− 18.65
	X42	1	711.39	711.39	41.96	< 0.0001*	16.96
	X52	1	454.27	454.27	26.80	< 0.0001*	13.55
Error effect	Lack of fit	22	444.75	20.22	3.02	0.0688
	Pure error	7	46.88	6.70
R2	0.9888	Std. dev	4.12
Adj R2	0.9810	Mean	60.78
Pred R2	0.9642	C.V. %	6.77
Adeq Precision	34.38	PRESS	1568.50

*Significant values, F Fishers's function, P Level of significance, C.V Coefficient of variation.