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. 2020 Aug 10;11:3989. doi: 10.1038/s41467-020-17798-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — (a) Representative flow cytometry analysis of Prdm1^fl/fl (Ctrl) and Cd19^Cre/+Prdm1^fl/fl (BcKO) B cells 72 h after LPS inoculation. (b) ERCC normalized total mRNA content for samples from the indicated divisions sorted from (a). c Bar plot showing the number of differentially expressed genes (DEG) for the indicated division between Ctrl and BcKO B cells. d Principal component analysis of samples from (b) using all DEG. The location of cells in each division is labeled. Circles denote 99% confidence intervals for samples in each division. e Bar plot of expression of select genes from divisions 0, 1, 3, 5, 8^– and for Ctrl 8⁺. FPKM fragments per kilobase per million. Data in (b, e) represent mean ± SD and asterisk indicates statistical significance (FDR < 0.05) as determined by edgeR⁵⁹. Data were derived from 2 to 4 individual mice as follows: Ctrl division 0 (n = 2), 1 (n = 2), 3 (n = 3), 5 (n = 3), 8− (n = 2), and 8+ (n = 3); BcKO division 0 (n = 3), 1 (n = 4), 3 (n = 4), 5 (n = 4), 8− (n = 4). Source data are provided as a Source Data file.