Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 10;11:3989. doi: 10.1038/s41467-020-17798-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Scatter plot showing the expression log₂ fold change (log₂FC) versus the log₂FC in SCENIC activity score between cluster 3 versus 7 for each transcription factor. Gray line represents linear regression with significance determined by one-way ANOVA. Pearson’s r correlation is indicated. bt-SNE plot (left) from Fig. 4a and violin plot (right) showing the MAGIC gene expression data for the indicated gene. For violin plots, dots represent mean and lines represent first and third quartile ranges. ct-SNE plot showing mean MAGIC expression levels for BATF target genes determined by SCENIC. d Heatmap showing expression of 46 BATF target genes along pseudotime in WT cells. For each gene, the mean MAGIC expression level is normalized to the maximal value. e Pie chart representing the percentage of BATF target genes containing either the canonical AP-1 or composite IRF:AP-1 (AICE) motif. ft-SNE plot (left) and violin plot (right) showing the MAGIC gene expression data for the indicated gene. For violin plots, the dots represent mean and lines represent first and third quartile ranges. g Representative flow cytometry (left) and geometric mean fluorescence intensity at each cell division (right) for intracellular staining of IRF4 levels versus CTV in LPS-responding B cells 72 h after LPS inoculation. Division 7/8 cells are subdivided into CD138 negative (−) and positive (+). Data represent mean ± SD and statistical significance determined by two-tailed Student’s t test. Data represent two independent experiments of nine mice. ht-SNE plot of 6903 Cd19^Cre/+Irf4^fl/fl (IRF4^–/–) cells responding to LPS showing the location of six distinct cell clusters. Data represent combined cells from two independent mice. it-SNE plot of pseudotime ordered cells from (h) showing the location of cells from each cluster. jt-SNE plot from (h) showing the annotation of each cell with a WT cluster from Fig. 4a. kt-SNE plot from (h) showing mean MAGIC expression levels for BATF target genes as in (c). lt-SNE plot from (h) showing mean MAGIC expression for Leading Edge genes from the indicated gene set (see Fig. 5e). Source data are provided as a Source Data file.