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. 2020 Aug 4;11:1170. doi: 10.3389/fpls.2020.01170

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Copyright © 2020 Carro-Huerga, Compant, Gorfer, Cardoza, Schmoll, Gutiérrez and Casquero

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CSLM Control plants and plants inoculated with Trichoderma sp. T154::tdTom3 (arrowhead) after 6 weeks. (A) Parenchyma zone without any sign of fluorescence. (B) Inoculation point showing different tissues free of fungi. (C) Xylem zone and occlusion close to the injured zone without any sign of fluorescent fungi. (D, E) Inoculation point colonized by spores and its 3D modelling identifying clearly all spores. (F) Zoom of the previous picture for showing a big group of Trichoderma sp. T154::tdTom3 alive spores dispersed randomly inside tissues. (G, H) Inside dead tissues is possible to identify Trichoderma sp. T154::tdTom3 mycelium. (I) Xylem section showing spores of Trichoderma sp. T154::TdTom3 inside it. Fib.: fibers, Inoc. point: inoculation point, Metaxyl.: metaxylem, xyl.:xylem. Representative pictures of biological replicates (12) are presented in this figure.