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. 2020 Aug 4;11:853. doi: 10.3389/fgene.2020.00853

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Copyright © 2020 Liu, Zhu, Ho, Shi, Li, Wang, Chan and Cheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Marker gene analysis. Marker gene expression of the WT and miR-430^–/– mutant was analyzed at shield stage (A), 75% epiboly (B), 6-somite (C,D), and 36 hpf (E). Marker genes of axis formation (A), germ layer formation (B), organ progenitor formation, (C) neuronal pattern (D), and heart formation (E) were analyzed in the miR-430^–/– mutants. The ratios of the observed phenotypes were indicated. FB, forebrain; MHB, midbrain hindbrain boundary; OV, otic vesicle; OP, optic placode; R3, rhombomere 3; R5, rhombomere 5.