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. 2020 Aug 4;11:853. doi: 10.3389/fgene.2020.00853

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Copyright © 2020 Liu, Zhu, Ho, Shi, Li, Wang, Chan and Cheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcriptome analysis. Total mRNAs of embryos from WT, miR-430^–/– mutant and rescued groups were collected at shield stage for transcriptome sequencing. (A) Identification of miR-430-regulated genes. (B) MiR-430 binding site analysis. The ratios indicate the numbers of genes with canonical sites of the indicated miRNA in their 3′UTR/total number of analyzed genes. The miRNA binding sites were predicted by TargetScanFish 6.2. (C) GO analysis of the miR-430 regulated genes, performed by the DAVID system. (D) The expression of lft2 in the transcriptome of the WT, miR-430^–/– and rescued embryos. (E) Q-PCR analysis of lft2 mRNA expression levels in the WT, miR-430^–/– and rescued group. Data are expressed as mean values ± S.E.M (n = 4). (F) MO knockdown of lft2 partially rescued the miR-430-deficient phenotypes. The embryos were collected at 75% epiboly and 36 hpf for WISH analysis of marker gene expression.