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. 2020 Aug 4;8:740. doi: 10.3389/fcell.2020.00740

FIGURE 3.

FIGURE 3

Bach1 inhibitors attenuate RANKL-mediated osteoclastogenesis. Representative photographs of TRAP staining are shown of randomly selected field: (A) control, (B) RANKL (100 ng/ml), (C) RANKL (100 ng/ml) + HPP-A (400 nM), (D) RANKL (100 ng/ml) + HPP-B (400 nM), (E) RANKL (100 ng/ml) + HPP-C (400 nM), and (F) RANKL (100 ng/ml) + HPP-E (400 nM). Arrowhead indicates TRAP-positive multinucleated cells. Scale bars; 100 μm. (G) Mean number of TRAP-positive multi-nucleated cells differentiated from RAW 264.7 cells at 400 nM (open bar) and 80 nM (close bar). The data shown are representative of three independent experiments performed in triplicate. ∗P < 0.05 vs. RANKL alone. †P < 0.05 between the groups. NS: no significant difference compared with the control.