FIGURE 3.

FAM83H implicates in ESCC progression and is regulated by FAM83H‐AS1 at mRNA and protein level. A, The interfering efficiency against FAM83H analysed by qRT‐PCR method. B, Cell proliferation was assessed using MTS assay with silenced FAM83H in Kyse150 and TE1 cells. C, Transwell migration and D, invasion assays were carried out with silenced FAM83H in Kyse150 and TE1 cells (magnification, ×200). E, and F, The influence of FAM83H‐AS1 on FAM83H mRNA and protein level detected by qRT‐PCR and Western blot assays. G, The influence of FAM83H on FAM83H‐AS1 expression analysed by qRT‐PCR method. Data are shown as mean ± SD; *P < .05 and **P < .01