FIGURE 4.

FAM83H‐AS1 is up‐regulated in TGF‐β‐treated Eca109 cells and contributes to EMT process. A, Cell morphology in TGF‐β‐treated or untreated Eca109 cells. B, Relative expression of EMT‐related markers was detected in TGF‐β‐treated Eca109 cells. C, Relative expression of FAM83H‐AS1 was assessed in TGF‐β‐treated Eca109 cells. D, and E, The regulation of FAM83H‐AS1 on EMT‐related markers detected by qRT‐PCR method. F, The effect of FAM83H‐AS1 on EMT‐related markers observed by Western blot assay. Data are shown as mean ± SD; *P < .05 and **P < .01