FIGURE 5.

MiR‐10a‐5p contains binding sites to FAM83H‐AS1 and functions as tumour suppressor role. A, The sequence of predicted (wild‐type) and mutated (mutant type) binding sites for miR‐10a‐5p on FAM83H‐AS1. The red nucleotides are the seed sequences of miR‐10a‐5p. B, Relative expression of miR‐10a‐5p in 67 pairs of ESCC tissues and corresponding normal tissues confirmed by qRT‐PCR method. C, Relative expression of miR‐10a‐5p in four human oesophageal cancer cell lines detected by qRT‐PCR method. Pools: average expression in 10 normal tissues was used as normal control. * Compared with the pools. D, Relative expression of miR‐10a‐5p in different subgroups. E, The correlation between FAM83H‐AS1 and miR‐10a‐5p expression. F, Relative expression of miR‐10a‐5p detected by transfection with miR‐10a‐5p mimics or inhibitor. G, MTS assay and H, clone formation assay were conducted by transfection with miR‐10a‐5p mimics or inhibitor. I, Transwell migration and J, invasion assays were performed by transfection with miR‐10a‐5p mimics or inhibitor (magnification, ×200). Data are shown as mean ± SD; *P < .05 and **P < .01