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. 2020 Jul 12;24(16):9446–9456. doi: 10.1111/jcmm.15616

FIGURE 5.

FIGURE 5

Role of Nrf2 in EA‐mediated DA neuroprotection. C6 cells were treated with Nrf2 siRNA (50 nmol/L). After 6 h of transfection, the transfection solution was removed and cells were rinsed with PBS. The silence efficiency was validated by real‐time RT‐PCR and Western blotting (A). Then, C6 cells conditionally silencing Nrf2 were seeded in transwells upper chamber followed by MN9D cells seeded in the lower chamber to establish MD9D‐C6 co‐cultures and then treated with EA for 30 min and ROT for another 24 h. DA neuronal damage was determined by TH‐positive neuronal number counting (B) and TH protein expression detection (C). Scale bar = 100 μm. Results were mean ± SEM from three independent experiments performed in triplicate. *P < 0.05 compared with the control cultures, # P < 0.05 compared with ROT‐treated cultures, △ P < 0.05 compared with ROT + EA cultures