FIGURE 3.

WWP2 promotes levels of STAT3‐K685 acetylation and STAT3‐Y705 phosphorylation. A and B, HA‐WWP2 and WWP2 siRNA were transfected into HAVSMCs. STAT3 was isolated by co‐immunoprecipitation, and STAT3‐Y705 phosphorylation levels were assessed with anti‐STAT3‐Y705 antibody. C and D, HA‐WWP2 and WWP2 siRNA were transfected into HAVSMCs. STAT3 was isolated by co‐immunoprecipitation, and STAT3‐K685 acetylation levels were assessed with anti‐STAT3‐K685 antibody. E and F, HA‐WWP2 and WWP2 siRNA were transfected into HAVSMCs with or without treatment of 10⁻⁶ mol/L AngII after serum‐free medium starvation. STAT3‐K685 acetylation and STAT3‐Y705 phosphorylation levels were assessed with anti‐STAT3‐K685 and anti‐STAT3‐Y705 antibodies