FIGURE 5.

Effect of ATX on Nrf2 translocation in human SH‐SY5Y cells. A, SH‐SY5Y cells were treated with different concentrations of ATX (5, 10 and 20 μmol/L) for 24 h. B, SH‐SY5Y cells were treated with ATX (20 μmol/L) with different time durations (6, 12, 24 and 48 h). The nuclear and cytosolic levels of Nrf2 were determined by Western blot analysis. Lamin B1 and β‐actin were used as nuclear and cytosolic loading controls, respectively. Results were expressed as mean ± SD (n = 3). *P < .05, **P < .01 vs Control group. Significance was determined by one‐way analysis of ANOVA followed by Dunnett's test