FIGURE 1.

Characterization of glycation of FN by MGO. FN (1 mg/mL) was incubated with MGO (0, 0.1, 1, 10 and 50 mM) at 37°C for 7 days. A, The samples were separated by SDS‐PAGE, and FN was detected with immunoblotting. B, AGEs were also immunoblotted on the same blots after stripping. C, The fluorescence intensity of MGO‐FN (50 mM MGO) was measured at 370/440 nm in the fractions. Results represent the mean ± SD for triplicate determinations. **P < .01