FIGURE 6.

Glycation of FN inhibits VEGF‐induced angiogenesis in Matrigel plug assay. Matrigel supplemented with FN, FN and VEGF, MGO‐FN or MGO‐FN and VEGF was injected subcutaneously in mice for 14 days. Then, the Matrigel plugs were harvested, fixed and sectioned. A, The plugs were stained with haematoxylin and eosin, and the representative images are shown. B, The endothelial cells were labelled by immunochemical staining of CD31, and the representative images of CD31‐positive microvessels (arrows) in Matrigel plugs are shown. Scale bar is 100 μm. Quantitative assessment of microvessel formation was performed, and all data shown are mean ± SD. ***P < .001