Figure 3.

Exogenous H₂S decreased oxidative stress induced diabetic atrophied muscle. A, B, Superoxide generation was measured in frozen muscle sections and C2C12 myoblasts of control and db/db and db/db mice with the treatment of NaHS and C2C12 myoblasts treated by NAC and PPG using dihydroethidium staining. C, D, Expression of SOD and CAT in animal and cellular model were tested by Western blot. E, F, The activities of SOD and CAT were measured using spectrophotometric‐based assay. G, H, Western blotting analyses the expression of protein markers of ER stress in animal and cellular model. Values are mean ± SD. n = 5, *P < 0.05, **P < 0.01, ***P < 0.001. Significantly different from corresponding control values. H₂S, hydrogen sulphide