Excluded studies	Reason for exclusion
Baker DC, Keeler RF and Gaffield WP, 1988. Mechanism of death in Syrian hamsters gavaged potato sprout material. Toxicologic Pathology, 16, 333–339.	In the study, no GA concentrations were determined
Bell DP, Gibson JG, McCarroll AM and McClean GA, 1976. Embryotoxicity of solanine and aspirin in mice. Journal of Reproduction and Fertility, 46, 257–259.	i.p. study. Although the authors call it solanine, in fact a‐solanine was used (standard from Sigma)
Caldwell KA, Grosjean OK, Henika PR and Friedman M, 1991. Hepatic ornithine decarboxylase induction by potato glycoalkaloids in rats. Food Chemistry and Toxicology, 29, 531–535.	Excluded because additional application of dexamethasone in all treatment groups, no proper control
Candeias MF, Abreu P, Pereira A and Cruz‐Morais J, 2009. Effects of strictosamide on mouse brain and kidney Na+, K+‐ATPase and Mg2+‐ATPase activities. Journal of Ethnopharmacology, 12, 117–122.	Strictosamide is not a potato GA
Chaube S, Swinyard CA and Daines RH, 1973. Failure to induce malformations in fetal rats by feeding blighted potatoes to their mothers. Lancet, 1, 329–330.	In the study, no GA concentrations were determined
Crawford L and Myrh B, 1995. A preliminary assessment of the toxic and mutagenic potential of steroidal alkaloids in transgenic mice. Food Chemistry and Toxicology, 33, 191–194.	A preliminary study without proper controls and without value for the mutagenicitiy assessment
Friedman M, Rayburn JR and Bantle JA, 1992. Structural relationships and developmental toxicity of Solanum alkaloids in the frog embryo teratogenesis assay‐Xenopus. Journal of Agricultural and Food Chemistry, 40, 1617–1624.	Studies in Xenopus are not informative for the risk assessment
Gupta AK, Ganguly P, Majumder UK and Ghosal S, 2009. Antidiabetic and antihyperlipidaemic effects of Solanum Xanthocarpum total extract in alloxan induced diabetic rats. Pharmacologyonline, 1, 484–497.	In the study, no GA composition and concentration were determined
Hellenäs KE, Cekan E, Slanina P and Bergman K, 1992. Studies of embryotoxicity and the incidence of external malformations after continuous intravenous infusion of α‐chaconine in pregnant rats. Pharmacology and Toxicology, 70, 381–383.	i.v. excluded from the consideration in the experimental animal section, except in the acute toxicity for comparison with the oral route
Hornfeldt CS and Collins JE, 1990. Toxicity of nightshade berries (Solanum dulcamara) in mice. Clinical Toxicology, 28, 185–192.	In the study, no GA composition and concentration were determined
Ji Y, Sun J and Lang L, 2011. Analysis of testicular toxicity of solanine in mice. Advanced Materials Research, 282, 195–200.	i.p. administration
Patel VB, Rathod IS, Patel JM and Brahmbhatt MR 2010. Anti‐urolithiatic and natriuretic activity of steroidal constituents of Solanum xanthocarpum. Der Pharma Chemica, 2, 173–176.	No proper methodical description of the experiments and no proper description of isolation and purification procedure. Purity of the tested substances is highly questionable
Peičev P, Nikiforov N and Rusev R, 1967. Pharmacological properties of glycoalkaloids, obtained from Solanum tuberosum L. Experimental Medicine and Morphology. VI, 1.	Extract used of Herba Solani (leaves of S. tuberosum), purity of isolated GA is not described, documentation is incomplete
Poswillo DE, Sopher D and Mitchell S, 1972. Experimental induction of foetal malformation with ‘‘blighted’’ potato: a preliminary report. Nature, 239, 462–464.	In the study, no GA composition and concentration were determined
Poswillo DE, Sopher D, Mitchell SJ, Coxon DT, Curtis RF and Price KR, 1973. Further investigations into the teratogenic potential of imperfect potatoes. Symposium on Potatoes and Birth defects, 8, 339–347.	Only TGA content reported. Reported TGA content is much lower than generally found in (healthy) potato tubers, indicating an error in the analytical methodology used
Schwarz A, Pinto E, Haraguchi M, de Oliveira CA, Bernardi MM and de Souza Spinosa H, 2007. Phytochemical study of Solanum lycocarpum (St. Hil) unripe fruit and its effects on rat gestation. Phytotherapy Research, 21, 1025–1028.	Extracts of S. lycocarpum were tested, which is not a food plant
Sharma RP, Willhite CC, WU MT and Salunkhe DK, 1978. Teratogenic potential of blighted potato concentrate in rabbits, hamsters and miniature swine. Teratology, 18, 55–62.	In the study, no GA composition and concentration were determined
Wang XG, 1993. Teratogenic effect of potato glycoalkaloids. Zhonghua Fu Chan Ke Za Zhi, 28, 121–122.	Abstract only. Intra‐abdominal administration. Article in Chinese
Yoon DJ and Kirkowski A, 1979. Effects of dietary Solanaceous potatoes on the serum, calcium, phosphorus, magnesium and hydroxyproline levels in rats. Korean Journal of Animal Science, 21, 401–406.	In the study, no GA composition and concentration were determined
Allen et al., 1977. Teratogenicity studies on late blighted potatoes in nonhuman primates (Macaca mulatta and Saguinus labiatus). Teratology, 15, 17–23.	Only TGA content reported. Reported TGA content is much lower than generally found in (healthy) potato tubers, indicating an error in the analytical methodology used
Rao MV, 1988. Effects of alcoholic extract of Solanum xanthocarpum seeds in adult male rats. Indian Journal of Experimental Biology, 26, 95–98.	Extract of Solanum xanthocarpum seeds. In the study, no GA composition and concentration were determined
Mali PC, Chaturvedi M and Dixit VP, 1996. Antispermatogenic activity of Solanum xanthocarpum S and W (50 percent EtOH‐extract) in rats. Journal of Phytological Research, 9, 13–17.	Extract of Solanum xanthocarpum root. In the study, no GA composition and concentration were determined