Fig. 9.

A is micrographs of whole brain stained with Black gold II, showing demyelination in the CA1 region (red arrow), the thalamus (black arrow), the habenula (yellow arrow) and the rostral commissure (purple arrow). The internal capsule (white arrow) appeared resistant to the demyelination. Distance of each section from the bregma is according to Paxinos and Franklin (2001). B depicts graphs showing the degree of myelination by densitometry of Black Gold II histochemistry expressed as optical density (OD) of the corpus callosum (upper and middle bands), the somatosensory and retrosplenial cortices. Micrographs in B denote the regions of concern (highlighted in a box), all from the control groups. Groups – Control (sterile water); V (Vanadium 3 mg/kg); V + M10 (Vanadium 3 mg/kg + MIMO2 10 mg/kg); M10 (MIMO2 10 mg/kg). Number of mice per group = 5. (*p < 0.05, **p < 0.01, ***p < 0.001).