Figure 2.
p66Shc Contributes to ECM Production during Liver Fibrosis and Is Modulated by CA
(A) p66Shc protein expression in rat livers; n = 3. ∗∗p < 0.01 versus the control group; #p < 0.05 versus the CCl4 group; ##p < 0.01 versus the CCl4 group. (B) p66Shc, α-SMA, and COL1A2 protein expression in LX-2 cells. LX-2 cells were treated with CA, PDGF-BB, SHC1 siRNA, or nothing; n = 3. ∗p < 0.05 versus the control group; ∗∗p < 0.01 versus the control group; #p < 0.05 versus the PDGF-BB group; ##p < 0.01 versus the PDGF-BB group. (C) Immunofluorescence images (×200) showing p66Shc (red) and α-SMA (green) expression in LX-2 cells. DAPI was used as a counterstain.