Fig. 6.
Quantification of MCP1 and RANTES in J774A.1 cells following treatment with HOCl and HOSCN. J774A.1 cells (1 × 106 cells) were treated with HOCl (50, 100 μM), HOSCN (100, 200 μM), and 100 μM HOCl with SCN− (50, 200 μM) for 1 h at 37 °C, before re-incubation in cell media for 3 h (black bars) or 24 h (grey bars). For intracellular cytokine determination, the concentrations of MCP1 (A) and RANTES (B) were measured in cell lysates by ELISA and normalized to total cell protein concentration. To assess extracellular release of cytokines, the concentrations of MCP1 (C) and RANTES (D) were measured in the cell media, after centrifugation to remove cell debris. The release of RANTES after 3 h re-incubation was below the limit of detection. Data are shown as the fold change compared to the non-treated control group. *, **, *** and **** show a significant difference (p < 0.05, 0.01, 0.001 and 0.0001, respectively) compared to the non-treated control (HBSS) group by 2-way ANOVA with a Dunnett's multiple comparison test.