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. 2020 Aug 10;10:13487. doi: 10.1038/s41598-020-70366-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Localization of the S1426I ATP7B mutant in HEK293 cells under low and high Cu conditions. (A) Multiple sequence alignment showing conservation of Ser1426Ile. (B) HEK293 cells were transfected (12 h) with WT or S1426I mutant ATP7B GFP-ATP7B (green) and treated for 3 h with basal medium (top), medium containing 100 µM of CuCl₂ (middle), or medium containing 25 µM of tetra-thiomolybdate (TTM) (lower panel). Trans-golgi network (TGN) marker, TGN46, in red and nuclei (DAPI) in blue. Co-localization is indicated in yellow. Green signals are from GFP fluorescence.