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. Author manuscript; available in PMC: 2021 Aug 20.
Published in final edited form as: Mol Cell Endocrinol. 2020 Jun 15;514:110911. doi: 10.1016/j.mce.2020.110911

Figure 2. (Single column). Effect of FSH glycoform preparations on CREB phosphorylation in porcine granulosa cells.

Figure 2. (Single column)

Granulosa cells were treated for 30 minutes with increasing concentrations of hFSH21 or hFSH24. Cell lysates were prepared and Western blot analysis was performed using phospho-Ser133-CREB (P-CREB) antibody, and β-actin antibodies. A, Fold increases in the phospho-CREB/Actin ratio; results are presented as means ± SEM; n = 3 separate experiments. #P < 0.05 and ##P < 0.01 compared with respective control; *P < 0.05 and **P < 0.01 compared with the other glycoform at the same concentration. B, representative Western blot analysis of CREB phosphorylation after hFSH glycoform treatment. Actin was used as an internal control.