Figure 4. (Single column). Effects of hFSH glycoform preparations at stimulating CRE-mediated transcriptional activity.
Granulosa cells were infected with an adenovirus expressing a CRE luciferase reporter (CRE-Luc) or pRL-Luc as an internal control. After 24 hours, granulosa cells were treated for 6 hours with either hFSH21or hFSH24 ranging from 0–100 ng/mL. Relative CRE-luciferase activity (rlu) was determined as described in the Methods. Bars represent means± SEM; n = 4 separate experiments. #P < 0.05 and ##P < 0.01 compared with control; *P < 0.05 and **P < 0.01 compared with the other glycoform at the same concentration.