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. Author manuscript; available in PMC: 2021 Aug 20.
Published in final edited form as: Mol Cell Endocrinol. 2020 Jun 15;514:110911. doi: 10.1016/j.mce.2020.110911

Figure 5. (Single column). Effect of FSH glycoform preparations on β-catenin phosphorylation in porcine granulosa cells.

Figure 5. (Single column)

Cells were treated with increasing concentrations of hFSH21 or hFSH24 for 30 min. Cell lysates were prepared for Western blot analysis using phospho-Ser552 and phospho-Ser675-β-catenin (P-β-catenin) antibodies, and actin antibodies. A, fold changes in the phospho-β-catenin Ser552/Actin ratio; B, fold changes in the phospho-β-catenin Ser675/Actin ratio; C, representative Western blot analysis of phosphorylation of β-catenin after FSH glycoform treatment. Actin was used as an internal control. Data are expressed as means ± SEM; n = 3 separate experiments. #P < 0.05 and ##P < 0.01 compared with respective control; *P < 0.05 and **P < 0.01 compared with the other glycoform at the same concentration.