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. Author manuscript; available in PMC: 2021 Aug 20.
Published in final edited form as: Mol Cell Endocrinol. 2020 Jun 15;514:110911. doi: 10.1016/j.mce.2020.110911

Table 1.

Antibodies used for Western blotting and microscopy.

Dilution ratio Species
Specificit y		 Source Supplier Cat. No
CREB 1:1000 Mouse Rabbit mAB Cell Signaling 9197L
Phospho-CREB 1:10001/1:2002 Mouse Rabbit pAB Cell Signaling 9198S
(Ser133)
Phospho-PKA 1:1000 Mouse Rabbit pAB Cell Signaling 9624S
Substrate(RRXS */T *)
β-catenin 1:1000 Mouse pAB Cell Signaling 2698S
Phospho-P-catenin (Ser552) 1:1000 Mouse Rabbit mAB Cell Signaling 9566S
Phospho-P-catenin (Ser675) 1:1000 Mouse Rabbit mAB Cell Signaling 4176S
HSD3B1 1:1000 Mouse Rabbit mAB A gift from Dr. Ian Mason
Actin 1:5000 Bovine Mouse mAB Sigma-Aldrich A5441
α-tubulin 1:200 Bovine Mouse mAB Abcam Ab7291
HRP-linked 1:10000 Anti-rab
bit		 Jackson ImmunoResearch 111035144
HRP-linked 1:10000 Anti-mo
use		 Jackson ImmunoResearch 115035205
Alexa Fluro 488 1:500 Anti-mo
use		 Invitrogen A21202
Alexa Fluro 594 1:500 Anti-rab
bit		 Invitrogen A11032
1

Dilution used for Western blotting.

2

Dilution used for Confocal Microscopy.