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. Author manuscript; available in PMC: 2020 Aug 11.
Published in final edited form as: Cell Rep. 2020 Jun 23;31(12):107804. doi: 10.1016/j.celrep.2020.107804

Figure 3. H2B-FT Color Profile Reflects Cell Proliferation Rate In Vitro.

Figure 3.

(A) Proliferation of H2B-FT MEFs after transduction with c-Myc or empty vector control (EV). Error bars: SD across n = 4 culture wells.

(B) Cellular BR at 25 and 70 h post-transduction. Box plots represent median and interquartile range (IQR); whiskers, 5th–95th percentiles. p = 0.295 (25 h) and p < 0.0001 (70 h) on the basis of Mann-Whitney test with 99% confidence interval (CI).

(C) Representative blue versus red intensity of individual cells at 70 h post transduction. Flow cytometry-style gates define “red” and “blue” populations (see also Figures S3A and S3B).

(D) Percentage of cells within gates defined in (C). Percentages do not total 100%, as many cells lie between red and blue gates. Error bars show SD across n = 4 culture wells. p = 0.0035 (red cells) and p = 0.0498 (blue cells) on the basis of Student’s t test with Welch’s correction, 95% CI. dF = 4.057 (red cells) and dF = 3.221 (blue cells).

(E) Cell cycle length distributions in each gate as measured by image-based tracking. Cell cycle length refers to the time interval between consecutive mitoses.

(F) Correlation between cell cycle length and BR. All trackable cells (n = 220) from c-Myc and EV conditions are plotted. Spearman correlation coefficient = 0.7648; p < 0.0001 with 95% CI.

n values in (B) and (E) refer to the numbers of single cells analyzed.