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. Author manuscript; available in PMC: 2020 Aug 11.
Published in final edited form as: Cell Rep. 2020 Jun 23;31(12):107804. doi: 10.1016/j.celrep.2020.107804

Figure 5. The Proliferative Landscape of Live Hematopoietic Cells as Captured by the H2B-FT Reporter.

Figure 5.

(A) Representative flow cytometry plots of LKS and GMP cells from mouse BM reconstituted by HSPCs virally expressing H2B-FT.

(B) Data from (A) plotted as BR distribution, with WBM BR added.

(C) Flow cytometry plots of myeloid (Mac1+) and erythroid (Ter119+) cells from the mouse in (A) and (B).

(D) BR distribution of data in (C). Gates in (A) and (C) excluded the non-transduced (H2B-FT−) cells.

(E) Targeting strategy for the iH2B-FT mouse allele.

(F) DAPI/EdU profiles of GMPs versus early erythroid cells after 2 h EdU labeling in vivo.

(G) BR distributions in LKS, GMPs, and early erythroid cells.

(H) Percentage of myeloid (Mac1+), B cell (B220+), and T cell (CD3+) cells in peripheral blood of healthy iH2B-FT mice versus those crossed with MLL-ENL (n = 3 mice per group). All mice were treated with Dox ≥8 days prior to analysis. Error bars represent SD; p = 0.0090 (Mac1+), p = 0.0072 (B220+), and p = 0.7629 (CD3+) on the basis of Student’s t test with 95% CI, dF = 4.

(I) Representative BR profiles of the PBMN subsets in (H).