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. Author manuscript; available in PMC: 2020 Aug 11.
Published in final edited form as: Cell Rep. 2020 Jun 23;31(12):107804. doi: 10.1016/j.celrep.2020.107804

Figure 6. H2B-FT Profile Is Consistent with Relative Turnover Rates in Solid Tissue Sections.

Figure 6.

(A) H2B-FT appearance in representative frozen sections from adult and embryonic iH2B-FT mice. Top: kidney. Middle: gastric glands in two orientations, longitudinal (pylorus I) and oblique/cross-sectional (pylorus II). L, lumen; S, submucosa. Bottom: neocortex of an E17.5 embryo. Dotted line indicates lateral ventricle (LV). See Figure S6 for single-channel images.

(B) BR histogram for each image in (A) was subdivided into five populations of increasing BR (P1–P5).

(C) Left, a heatmap corresponding to P1–P5 in (B) projected onto images from (A). Right: the same regions after DAPI/EdU labeling.

(D) Zoomed-in view of pyloric regions boxed in (C). Arrow: base of a gastric gland.

(E) Tiled images of an E17.5 embryonic brain hemisphere. Left: H2B-FT. Middle: heatmap of BR binned as in (B). Right: same region after DAPI/EdU labeling. Dotted line indicates LV. IZ, intermediate zone; CP, cortical plate; MZ, marginal zone; Th, thalamus.

(F) BR distributions from all tissues in (A). “Pylorus” includes combined data from both orientations (pylorus I and II, A).

Scale bars, 200 μm (A and C), 80 μm (D), and 500 μm (E). Full-size images are shown in Data S3B–S3H.