| Antibodies |
|
|
| Biotin rat anti-mouse Ly-6G/6C Clone RB6-8C5 |
BD Biosciences |
Cat#553125; RRID:AB_394641 |
| Biotin hamster anti-mouse CD3e Clone 145-2C11 |
BD Biosciences |
Cat#553060; RRID:AB_394593 |
| Biotin rat anti-mouse CD45R/B220 Clone RA3-6B2 |
BD Biosciences |
Cat#553086; RRID:AB_394616 |
| Biotin rat anti-mouse CD11b Clone M1/70 |
BD Biosciences |
Cat#553309; RRID:AB_394773 |
| Biotin rat anti-mouse Ter119 |
BD Biosciences |
Cat#553672; RRID:AB_394985 |
| Biotin rat anti-mouse CD8a Clone 53-67 |
BD Biosciences |
Cat#553029; RRID:AB_394567 |
| Biotin rat anti-mouse CD4 Clone GK1.5 |
BD Biosciences |
Cat#553728; RRID:AB_395012 |
| APC rat anti-mouse CD117 Clone 2B8 |
BD Biosciences |
Cat#553356; RRID:AB_398536 |
| FITC rat anti-mouse Ly6A/E (Sca-1) Clone E13-161.7 |
BD Biosciences |
Cat#553335; RRID:AB_394791 |
| PerCP Streptavidin |
BD Biosciences |
Cat#554064; RRID:AB_2336918 |
| PE-Cy7 rat anti-mouse CD16/32 Clone 93 |
Thermo Fisher Scientific |
Cat#25-0161-82; RRID:AB_469598 |
| Alexa Fluor rat 700 anti-mouse CD34 Clone RAM34 |
BD Biosciences |
Cat#560518; RRID:AB_1727471 |
| APC rat anti-mouse CD11b Clone M1/70 |
Thermo Fisher Scientific |
Cat#17-0112-83; RRID:AB_469344 |
| FITC rat anti-mouse CD45R/B220 Clone RA3-6B2 |
BD Biosciences |
Cat#553087; RRID:AB_394617 |
| PE-Cy7 rat anti-mouse CD3 Clone 17A2 |
BioLegend |
Cat#100219; RRID:AB_1732068 |
| FITC rat anti-mouse CD71 Clone C2 |
BD Biosciences |
Cat#553266; RRID:AB_394743 |
| PE-Cy7 rat anti-mouse Ter119 |
Thermo Fisher Scientific |
Cat#25-5921-81; RRID:AB_469660 |
| FITC rat anti-mouse Ly6C Clone AL-21 |
BD Biosciences |
Cat#553104; RRID:AB_394628 |
| PE-Cy7 rat anti-mouse Ly6G Clone 1A8 |
BD Biosciences |
Cat#560601; RRID:AB_1727562 |
| Streptavidin MicroBeads |
Miltenyi Biotec |
Cat#130-048-101 |
| Bacterial and Virus Strains |
|
|
| pFast-FT-N1 |
Dr. Vladislav Verkhusha (Subach et al., 2009) |
RRID:Addgene_31910 |
| pMedium-FT-N1 |
Dr. Vladislav Verkhusha (Subach et al., 2009) |
RRID:Addgene_31911 |
| pSlow-FT-N1 |
Dr. Vladislav Verkhusha (Subach et al., 2009) |
RRID:Addgene_31912 |
| pFU-TetO-Gateway-PGK-Puro |
Dr. Jun Lu (Guo et al., 2012) |
N/A |
| pSCMV |
Dr. Jun Lu (Guo et al., 2012) |
N/A |
| PGK-H2B-mCherry |
Dr. Mark Mercola (Kita-Matsuo et al., 2009) |
RRID:Addgene_21217 |
| pMSCV-IRES-Blue FP |
Dr. Dario Vignali |
RRID:Addgene_52115 |
| MSCV-IRES-GFP |
Dr. Jun Lu (Lu et al., 2008) |
N/A |
| LZRS-c-Myc-IRES-GFP |
Dr. Sebastian Nijman |
N/A |
| pFU-TetO-H2B-FT-Fast-PGK-Puro |
This Publication |
N/A |
| pFU-TetO-H2B-FT-Medium-PGK-Puro |
This Publication |
N/A |
| pFU-TetO-H2B-FT-Slow-PGK-Puro |
This Publication |
N/A |
| pSCMV-H2B-FT-Fast |
This Publication |
N/A |
| pSCMV-H2B-FT-Medium |
This Publication |
N/A |
| pSCMV-H2B-FT-Slow |
This Publication |
N/A |
| p2Lox |
Dr. Michael Kyba (Iacovino et al., 2011) |
RRID:Addgene_34635 |
| pT3-Neo-EF1a-GFP |
Dr. Martin Bonamino (Chicaybam et al., 2017) |
RRID:Addgene_69134 |
| pCMV(CAT)T7-SB100 |
Dr. Zsuzsanna Izsvak (Mátés et al., 2009) |
RRID:Addgene_34879 |
| pT3-EF1a-H2B-FT-Fast |
This Publication |
N/A |
| pT3-EF1a-H2B-FT-Medium |
This Publication |
N/A |
| pT3-EF1a-H2B-FT-Slow |
This Publication |
N/A |
| Biological Samples |
|
|
| Primary neomycin resistant feeder MEFs, Mitomycin C treated |
Millipore Sigma |
Cat#PMEF-N |
| Chemicals, Peptides, and Recombinant Proteins |
|
|
| ESGRO mLif Medium Supplement |
Millipore Sigma |
Cat#ESG1107 |
| Recombinant murine IL-3 |
PeproTech |
Cat#213-13 |
| Recombinant murine SCF |
PeproTech |
Cat#250-03 |
| Recombinant murine TPO |
PeproTech |
Cat#315-14 |
| Recombinant human Flt3 Ligand |
PeproTech |
Cat#300-19 |
| Doxycycline hyclate |
Sigma Aldrich |
Cat#D9891; CAS ID 24390-14-5 |
| Retinoic acid |
Sigma Aldrich |
Cat#R2625; CAS ID 302-79-4 |
| DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) |
Thermo Fisher Scientific |
Cat#D1306; RRID:AB_2629482 |
| EdU (5-Ethynyl-2′-deoxyuridine) |
Cayman Chemical |
Cat#20518; CAS ID 61135-33-9 |
| 5-FU (5-Fluorouracil) |
APP Pharmaceuticals, LLC |
NDC 63323-117-10 |
| Critical Commercial Assays |
|
|
| Molecular Probes Click-iT Plus EdU Alexa Fluor 488 Imaging Kit Cat# C10637
|
Thermo Fisher Scientific |
Cat#C10637
|
| Stemgent AP Staining Kit II |
REPROCELL |
Cat#00-0055 |
| SuperScript III First-Strand Synthesis SuperMix |
Thermo Fisher Scientific |
Cat#18080400 |
| iQ SYBR Green Supermix |
Bio-Rad |
Cat#170-8882 |
| MACS LD Columns |
Miltenyi Biotec |
Cat#130-042-901 |
| QuadroMACS Separator |
Miltenyi Biotec |
Cat#130-090-976 |
| MACS MultiStand |
Miltenyi Biotec |
Cat#130-042-303 |
| Experimental Models: Cell Lines |
|
|
| HEK293T Cell Line |
ATCC |
Cat#CRL-3216; RRID:CVCL_0063 |
| HeLa Cell Line |
ATCC |
Cat#CRM-CCL-2; RRID:CVCL_0030 |
| BaF3 Cell Line |
RCB |
Cat#RCB0805; RRID:CVCL_0161 |
| A2lox.cre mESC Cell Line |
Dr. Michael Kyba (Iacovino et al., 2011) |
N/A |
| A2lox.cre H2B-FT-Fast mESC Cell Line |
This Publication |
N/A |
| A2lox.cre H2B-FT-Medium mESC Cell Line |
This Publication |
N/A |
| A2lox.cre H2B-FT-Slow mESC Cell Line |
This Publication |
N/A |
| A2lox.cre H2B-BFP mESC Cell Line |
This Publication |
N/A |
| A2lox.cre H2B-mCherry mESC Cell Line |
This Publication |
N/A |
| Experimental Models: Organisms/Strains |
|
|
|
Mus musculus: HPRT::iH2B-FT-Medium: B6.Cg- Gt(ROSA)26Sortm1(rtTA*M2)Jae Hprttm2(tetO-H2B/mediumFT)Sguo
|
This Publication |
MMRRC Cat#66959 |
|
Mus musculus: iMLL-ENL: Col1a1tm1(tetO-KMT2A/ELL)Dbry
|
Dr. David Bryder (Ugale et al., 2014) |
MGI ID 5825211 |
|
Mus musculus: Rosa26::rtTA: Gt(ROSA) 26Sortm1(rtTA*M2)Jae
|
Jackson Laboratory |
Cat#006965; RRID:IMSR_JAX:006965 |
|
Mus musculus: C57BL/6J |
Jackson Laboratory |
Cat#000664; RRID:IMSR_JAX:000664 |
| Oligonucleotides |
|
|
| Primers for cloning |
See Table S2
|
N/A |
| Primers for genotyping |
See Table S2
|
N/A |
| Primers for qPCR |
See Table S2
|
N/A |
| Software and Algorithms |
|
|
| ImageJ |
ImageJ (Schneider et al., 2012) |
RRID:SCR_003070 |
| MetaXpress Software |
Molecular Devices |
RRID:SCR_016654 |
| CellProfiler Image Analysis Software |
CellProfiler (Carpenter et al., 2006) |
RRID:SCR_007358 |
| FCS Express Image Cytometry |
De Novo Software |
RRID:SCR_016431 |
| FACSDiva Software |
BD Biosciences |
RRID:SCR_001456 |
| FlowJo Flow Cytometry Analysis |
FlowJo |
RRID:SCR_008520 |
| Prism Software |
GraphPad |
RRID:SCR_002798 |
