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. 2020 Aug 6;12:6779–6793. doi: 10.2147/CMAR.S264681

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© 2020 Zhang et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

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LINC00491 was upregulated in NSCLC, and LINC00491 silencing inhibited the biological activities of NSCLC cells. (A) GEPIA database was used to analyze LINC00491 expression in LUAD and LUSC. (B) RT-qPCR was performed to measure the LINC00491 expression in 57 pairs of NSCLC tissues and corresponding adjacent normal tissues. (C) The expression of LINC00491 in NSCLC cells was detected via RT-qPCR. A human nontumorigenic bronchial epithelial cell line BEAS-2B served as the control. (D) H522 and SK-MES-1 cells were transfected with specific siRNAs targeting the LINC00491 or si-NC and subjected to RT-qPCR analysis to determine transfection efficiency. (E, F) The proliferation and apoptosis were tested in H522 and SK-MES-1 cells after si- LINC00491 or si-NC transfection via CCK-8 assay and flow cytometry analysis. (G, H) Migration and invasion detection assays uncovered the migratory and invasive capacities of H522 and SK-MES-1 cells after LINC00491 downregulation. *P < 0.05 and **P < 0.01.

Abbreviations: LINC00491, long intergenic non-protein coding RNA 491; LUAD, in lung adenocarcinoma; LUSC, lung squamous cell carcinoma; NSCLC, non-small cell lung cancer; si-LINC00491, LINC00491-specific small interfering RNA; si-NC, negative control small interfering RNA; PI, propidium iodide.