TABLE 4.

Indicator species analysis of the prokaryotic ASV residing in the rhizosphere of canola in response to cropping diversification treatment in 2013 and 2016.

	2013			2016
Crop diversification1	Indicator species ASV	Closest identity	P value	Indicator species ASV	Closest identity	P value
Monoculture	ASV315	Thermomicrobiales	0,003**	ASV399	Acidobacteria sp.	0,002**
	ASV833	Paracoccus sp.	0,001**	ASV202	Thermomicrobiales	0,003**
	ASV380	Chloroflexi sp.	0,003**	ASV309	Actinobacteria sp.	0,002**
	ASV409	Haliangium sp.	0,010*	ASV576	Thermomicrobiales	0,007**
	ASV16	Intrasporangiaceae	0,026*	ASV276	Chloroflexi sp.	0,002**
	ASV1082	Thermomicrobiales	0,031*	ASV848	Micromonosporaceae	0,009**
	ASV280	Chthoniobacter sp.	0,042*	ASV119	Rhizobiaceae	0,013*
	ASV251	Chthoniobacter sp.	0,042*	ASV547	Chloroflexi sp.	0,003**
	ASV838	Rhodanobacteraceae	0,049*	ASV680	Tepidisphaera sp.	0,009**
				ASV334	Rhizobiaceae	0,015*
				ASV809	Planctomycetes	0,020*
				ASV315	Thermomicrobiales	0,022*
				ASV321	Thermomicrobiales	0,034*
				ASV460	Chloroflexi sp.	0,032*
				ASV60	Gaiella sp.	0,037*
				ASV142	Chthoniobacter sp.	0,030*
				ASV181	Tepidisphaerales	0,034*
				ASV629	Solirubrobacter sp.	0,025*
				ASV552	Pseudonocardia sp.	0,036*
				ASV137	Rubinisphaeraceae	0,045*
				ASV613	Chloroflexi sp.	0,043*
				ASV108	Thermomicrobiales	0,039*
				ASV463	Parafilimonas sp.	0,041*
				ASV227	Acidobacteria	0,045*
				ASV183	Chitinophagaceae	0,047*
				ASV1287	Pirellula sp.	0,047*
Low	ASV529	Rubrobacter sp.	0,015*	ASV501	Pyrinomonadaceae	0,001**
				ASV577	Streptosporangium sp.	0,008**
				ASV377	Lysobacter sp.	0,030*
				ASV697	Frankiales	0,036*
Medium				ASV1624	Acidobacteria	0,033*
High	ASV182	Pseudomonas sp.	0,002**
	ASV214	Gaiellales	0,009**
	ASV34	Gaiella sp.	0,012*
	ASV283	Haloactinopolyspora sp.	0,014*
	ASV498	Rhizobiales	0,014*
	ASV624	Bacteria	0,017*
	ASV93	Nitrososphaeraceae	0,016*
	ASV751	Acidobacteria	0,003**
	ASV59	Holophagae sp.	0,027*
	ASV24	Burkholderiaceae	0,027*
	ASV53	Nitrososphaeraceae	0,025*
	ASV248	Iamia sp.	0,029*
	ASV127	Acidobacteria	0,046*
	ASV262	Sphingomonas sp.	0,040*
	ASV302	Acidobacteria	0,048*

Indicator values (IndVal) were tested for significance by Monte Carlo permutation tests (α = 0.05, 999 permutations). ¹Monoculture, canola monoculture; Low, wheat-canola rotation; Medium, pea-barley-canola rotation; High, lentil-wheat-LL-pea-barley-RR. An empty row indicates the absence of indicator species. ²ASV in bold are indicator species found in 2013 and 2016 in the same crop diversification. Level of significance, *p < 0.05, **p < 0.01.