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. 2020 Aug 10;48(8):0300060520934254. doi: 10.1177/0300060520934254

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© The Author(s) 2020

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Silencing HOXA10-AS decreases OSCC proliferation ability. (a) HOXA10-AS was silenced by ASO in OSCC cell lines CAL27 and SCC9, and qPCR analysis of the HOXA10-AS expression levels were conducted. (b) CAL27 and SCC9 cells were transfected with ASO-control and ASO-HOXA10-AS; 72 hours later, cell growth curves were measured by CCK8 at the indicated time points. (c, d) Colony formation ability of CAL27 and SCC9 was inhibited when HOXA10-AS was silenced. Data are presented as mean ± standard deviation (SD) (n = 3) *P < 0.05, **P < 0.01, ***P < 0.001.HOXA10-AS, HOXA10 antisense RNA; OSCC, oral squamous cell carcinoma; ASO, antisense oligonucleotide; qPCR, quantitative PCR; CCK8, Cell Counting Kit-8.