Fig. 2.

Absolute quantification of intracellular CoA thioesters in l-lysine producing Corynebacterium glutamicum LYS-9 (left) and LYS-9 ∆sucCD (right) during growth on glucose. The data comprise the time profile of glucose, l-lysine, and cell concentration (a, b) and absolute levels of intracellular CoA thioesters (c, d). The yield for l-lysine reflects the major production phase, indicated by the gray area, and analyzed for free CoA and CoA thioesters at three time points. The data for these time points are shown in subfigures c and d. n = 3