Table 5.
Overall and stage-wise performance of methylation panels in stool for detection of colorectal neoplasms
| Gene panel | First author, year, Ref. No. | Country | Study group | No. | DNA methylation assay | Sn (%) | CRC stage | No. | Stage-specific Sn (%) | Sp (%) |
|---|---|---|---|---|---|---|---|---|---|---|
| MGMT, MLH1, and VIM (promoters) | Baek, 2009 [44] | South Korea |
NAA AA Ad CRC Cn |
30 22 52 60 37 |
MSP |
70 46 60 75 |
I/II III/IV |
35 25 |
71 80 |
87 |
| ITGA4, SFRP2, and CDKN2A (promoters) | Chang, 2010 [46] | Korea |
NAA AA Ad CRC Cn |
17 8 25 30 31 |
MSP |
65 88 72 70 |
I/II III/IV |
14 16 |
64 75 |
97 |
| SFRP2, GATA4/5, NDRG4 and VIM (promoters) | Lu, 2014 [49] | China |
CRC Cn |
56 40 |
MSP | 96 |
I/II III/IV |
32 24 |
94 100 |
65 |
| SFRP2 and WIF-1 (promoters) | Zhang, 2014 [50] | China |
NAA AA Ad CRC Cn |
20 15 35 48 30 |
MSP |
55 80 66 81 |
I/A II/B III/C IV/D |
7 20 14 7 |
86 80 71 100 |
97 |
| SFRP2, TFPI2, NDRG4, and BMP3 (promoters) | Park, 2017 [34] | South Korea |
AA CRC Cn |
36 35 40 |
MSP |
72 94 |
I/II III/IV |
17 18 |
88 100 |
55 |
| COL4A2 and TLX2 (promoter/exon 1) | Liu, 2019 [40] | China |
AA CRC Cn |
77 80 83 |
qMSP |
52 91 |
I/II III/IV |
43 37 |
86 97 |
98 |
Note: Stages I/II/III/IV as per UICC classification and stages A/B/C/D as per Dukes classification
Ref. reference, No. number, Sn sensitivity, Sp specificity, MSP methylation-specific polymerase chain reaction, qMSP quantitative methylation-specific real-time polymerase chain reaction, NAA non-advanced adenoma, AA advanced adenoma, Ad adenoma, Cn Control