Fig. 5.

Memantine suppresses microglia activation and neutrophil infiltration. a Immunofluorescence staining of Iba-1 (microglia marker) and Ly6G (neutrophil marker) in the spinal cords of IgG(AQP4+) mice treated with preventive memantine or vehicle at 1, 4, and 8 dpi or with therapeutic memantine or vehicle at 8 dpi. IgG(con) mice treated with vehicle were used as a sham control. Sections were counterstained with DAPI. Scale bar = 100 μm. b Relative intensity of Iba-1 immunofluorescence in the spinal cord. c Relative intensity of Ly6G immunofluorescence in the spinal cord. n = 5 per group. Data are mean ± SEM. One-way ANOVA with Tukey-Kramer post hoc test. ns, not significant; **p < 0.01; ***p < 0.001