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. 2020 Jul 30;11:2040622320940185. doi: 10.1177/2040622320940185

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© The Author(s), 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Scutellarein inhibits fibroblast differentiation. (A) Western blot analysis of the expression of α-SMA, collagen I and fibronectin in fibroblasts. HPF was stimulated by TGF-β1 and scutellarein for 48 h. Left panel: representative western blot results. Right panel: bar graphs of the western blot results. (B) Quantitative real-time polymerase chain reaction analysis of the expression of ACTA2, COL1A1, and FN in fibroblasts. HPF was stimulated by TGF-β1 and scutellarein for 48 h. (C) Immunofluorescence of α-SMA, collagen I. and fibronectin. HPF was stimulated by TGF-β1 and scutellarein for 48 h. (D) Western blot analysis of p-Smad2 and p-Smad3. HPF was stimulated by TGF-β1 and scutellarein for 1 h. Left panel: representative western blot results. Right panel: bar graphs of the western blot results. The statistical figures show the data for three replications. The experimental results are expressed as the means ± standard deviations. The data were analyzed by one-way analysis of variance.

^*p < 0.05; ^**p < 0.01; ^***p < 0.001.

HPF, human pulmonary fibroblast; Scu, scutellarein.