Fig. 2.
Exposure to Aβ for 24-h results in a dose-dependent increase in mitochondrial oxygen consumption. A) Line graph (mean ± SD) depicting the fold-change of oxygen consumption to baseline as a function of time from bEnd.3 cells exposed to vehicle and 9 μM Aβ1-42. Scatter plot(s) (mean ± SD) demonstrating oxygen consumption (pmol/min) by (B, F) basal respiration, (C, G) maximum respiration, (D, H) spare capacity, and (E, I) proton leak from (B–E) bEnd.3 cells and (F–H) primary cerebrovascular endothelial cells after 24 h exposure to vehicle, 5 μM Aβ1-42, 9 μM Aβ1-42, and 18 μM Aβ1-42. One-way ANOVA with Dunnett’s post analysis and linear trend analysis was used to determine the level of significance between the treatment groups (*p < 0.05; **p < 0.001; ***p < 0.0001), (B–E, n = 12 wells per group; F–I, control = 10, all other conditions = 6 wells per group).