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. 2020 Jul 29;20(4):57. doi: 10.3892/ol.2020.11918

Figure 2.

Figure 2.

Inhibition of autophagy prevents breast cancer cell death induced by erastin. (A) Western blotting and (B) quantification revealed that the changes in autophagy-related protein expression levels induced by erastin were suppressed by 3-MA. (C) Western blotting and (D) quantification revealed that Baf-A1 inhibited the downregulation of p62 and increased LC3B protein level. (E) LDH release assay demonstrated that erastin-induced cell death was significantly alleviated by pretreatment with 3-MA and Baf-A1. *P<0.01 vs. control untreated group. #P<0.01 vs. erastin only group. (F) LDH release assay demonstrated that knockdown of ATG5 significantly prevented erastin-induced cell death. (G) Western blotting and (H) quantification revealed that the changes in autophagy-related protein expression levels induced by erastin were suppressed by knockdown of ATG5 with siRNA. *P<0.01. LDH, lactate dehydrogenase; 3-MA, 3-methyladenine; Baf-A1, bafilomycin A1; ATG, autophagy related; LC3B, microtubule-associated proteins 1A/1B light chain 3B; siRNA, small interfering RNA.