Figure 6.

Persistence of viscerofugal nerve cell bodies in organ cultured preparations. A, Confocal micrograph showing biotinamide neuronal tracing from a rectal nerve trunk in a fresh preparation. The labeled nerve trunk is indicated by an asterisk. Numerous large axons and smaller varicose fibers can be seen coursing throughout the myenteric plexus in all directions. Viscerofugal nerve cell bodies were also labeled. B, Biotinamide neuronal tracing from rectal nerves after organ culture also revealed viscerofugal nerve cell bodies but a substantially reduced density of fine varicose fibers. Calibration, 100 μm.