Fig 3.

Ageing eliminates the protective effect of pre-exposure to volatile general anaesthetics and hyperoxia on mortality after traumatic brain injury (TBI). Effects on mortality of preexposure to isoflurane (ISO; purple), hyperoxia (green), and ISO in hyperoxia (striped) of 1- to 8-day-old (a) and 43- to 50-day-old (b) w1118 flies. Analogous experiments with sevoflurane (SEVO; yellow) in young (c) and old (d) flies. Note the significant reduction in mortality by both agents in young but not in old flies. Median is indicated by cross bar, mean by þ, and the range of values by whiskers. ∗∗P¼0.004, ∗∗∗P¼0.001, and ∗∗∗∗P<0.001. N¼12e14 for each group. Log-binomial regression analysis. Numeric values of means and confidence intervals are listed in Supplementary Table 6.