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Chemical and enzymatic hydrolysis of (SP/RP)-1. Reactions contained 10 mM HEPES-KOH buffer (pH 8.5) and 20% MeOH. The red line represents the time course for the chemical hydrolysis of 34 μM (RP/SP)-1 by 50 mM NaOH. The blue line represents the time course for hydrolysis of a single isomer of (RP/SP)-1 catalyzed by the addition of 29 nM In1W-PTE to 34 μM (RP/SP)-1, followed by hydrolysis of the other isomer by the addition of 50 mM NaOH.
