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. 2020 Jul 27;11:1668. doi: 10.3389/fmicb.2020.01668

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Copyright © 2020 Mishra, Kushveer, Khan, Pagal, Meena, Murali, Dhayalan and Venkateswara Sarma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of virulence factors under control of quorum sensing by 2,4-di-tertbutylphenol (2,4-DBP). (A) Visual qualitative assay for inhibition of Chromobacterium violaceum violacein production and Pseudomonas aeruginosa pyocyanin production by 2,4-DBP at indicated concentrations. (B) Quantitative estimation of violacein and pyocyanin production. The growth curve in the presence of 2,4-DBP, baicalein, and untreated control is shown in the inlet. (C) Inhibition of chitinase, LasA protease, LasA staphylolytic activity, and LasB elastase activities by 2,4-DBP at indicated concentrations. (D) Visual qualitative assay of inhibition of P. aeruginosa HCN production by 2,4-DBP at indicated concentrations. Bar diagrams represent mean percentage values of triplicates normalized with those obtained with untreated controls.