FIGURE 4.

Inhibition of P. aeruginosa biofilm formation and biofilm-associated factors by 2,4-di-tertbutylphenol (2,4-DBP) at the indicated concentrations. (A) Inhibition of P. aeruginosa biofilm formation, production of alginate, exo-polysaccharide (EPS), and rhamnolipid, and hydrophobicity. Bar values indicate mean percentages of triplicates normalized with those obtained with untreated controls. (B) Congo red agar visual qualitative assay for inhibition of biofilm formation. (C) Inhibition of eDNA production by baicalein and 2,4-DBP. The bar values show intensity of the bands as estimated by ImageJ software, from the agarose gel electrophoresis image shown below. (D) Fluorescence and light microscopic images of biofilms when treated with various concentrations of baicalein and 2,4-DBP. Two random representative images are shown for each treatment. The scale bars are equivalent to 25 μm.