Figure S3.

Logo Plot Representation of Mutation Effects on Binding and Expression, Related to Figure 3

Letter height indicates preference of each site for individual amino acids with respect to ACE2 binding (height above the center line) or RBD expression (height below the center line). Blue letters indicate the unmutated SARS-CoV-2 amino acid, and, where applicable, green letters indicate differences found in SARS-CoV-1. Yellow highlights mark residues that contact ACE2 in the SARS-CoV-2 or SARS-CoV-1 crystal structures. See the STAR Methods for details of how the amino acid preferences are calculated from the experimental measurements.