Skip to main content
. 2020 Aug 11;885:173442. doi: 10.1016/j.ejphar.2020.173442

Fig. 4.

Fig. 4

Effects of Norvaline (N), (−)-Epicatechin (E), and E + N on endothelial nitric oxide synthase (eNOS) protein estability in young (Y) and aged (A) endothelial cells. A, non-denaturing Western blot for the analysis of dimer/monomer eNOS. GAPDH was used to normalize levels and were compared vs. control (C) conditions. B, graphic representation of eNOS dimerization. Values of YC were set as 1. C, tetrahydrobiopterin (BH4): dihydrobiopterin (BH2) ratio was determined. D, illustrates representative Western blots images and relative changes of GCH1 protein levels normalized using GAPDH values. *P < 0.05 vs. YC, ^P < 0.05 vs. AC.