FIGURE 1.

OSTN alleviates DOX‐induced inflammation in vitro. H9C2 cells were treated with rhOSTN (5 µg/mL) or an equal volume of vehicle as the control in the presence or absence of DOX (1 µmol/L) for 24 h. A, The mRNA levels of inflammatory markers, Il‐1β and Tnf‐α (n = 6). B, The releases of IL‐1β, TNF‐α from H9C2 cells to the medium (n = 6). C and D, Representative western blot images and the quantitative results (n = 6). E, Immunofluorescence staining of T‐P65 in H9C2 cells (n = 6). Values represent the mean ± SD. *P < .05 versus PBS + Vehicle; ^# P < .05 versus DOX + Vehicle