FIGURE 1.

Clinical relevance of Tex in HCC. (A) Opal multicolor IHC staining with anti‐CD8, anti‐PD‐1, antibodies, and DAPI. We developed tissue array of 235 HCC patients and summarized clinical characteristics in Table 1. Each patient has more than 5‐year follow‐up. (B) Comparison of CD8⁺PD‐1⁺ T cells in tumor or peritumor. (C) Clinical relevance of Tex in HCC. TTR and OS curves were plotted in GraphPad Prism . The horizontal axis showed time after surgery and the vertical axis showed probability of recurrence or OS rates by the corresponding time. Two sets of data (low vs high) were represented by black and red curve, respectively.^low, low expression of indicated biomarkers;^high, high expression of indicated biomarkers. TTR, time to recurrence; DAPI: 4′,6‐diamidino‐2‐phenylindole; HCC: hepatocellular carcinoma; OS: overall survival; PD‐1: programmed death‐1; *P < .5; **P < .01; ***P < .001