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. 2020 Aug 11;6(3):00200-2020. doi: 10.1183/23120541.00200-2020

TABLE 2.

Primers and probes used for reverse transcriptase quantitative PCR gene expression analysis

Target gene Primer/probe Sequence (5′ to 3′) Amplicon size bp
pdgX PdgX F GCACTCGTCAGGGTGATAAA 109
PdgX R GATGAGCAAGTTGGAGTGAATG
PdgX Probe 6FAM-TGATCGTGTTCTCATTACCGGGCG-QSY
luxS LuxS F GAAGTGTCTGAGGCTTGGTTAG 103
LuxS R CCGTATAGCTTCCGCATTGATAG
LuxS Probe 6FAM-AGGTGTACAAGATCAAGCTTCTATTCCCG-QSY
dps Dps F GGGCTACCACTGGAACATTA 104
Dps R GTTCAGCCACCTCATCTACTC
Dps Probe 6FAM-TGCGTGTAATGCAAAGAAGTTTACGCC-QSY
hktE HktE F CAACAACCAGACTTTGCTGAAC 104
HktE R ACGAGGTTGGCTGAAGTAATC
HktE Probe 6FAM-TCGTATCAACGGAGATGCAGCACA-3IABkFQ
omp26 Omp26 F ACCGCACTTGCTTTAGGTATT 104
Omp26 R GCGATCTGGGTGATGTTGAA
Omp26 Probe JOE-TTGCTTCAGGCTATGCTTCCGCT-BHQ