TABLE 2.
Target gene | Primer/probe | Sequence (5′ to 3′) | Amplicon size bp |
pdgX | PdgX F | GCACTCGTCAGGGTGATAAA | 109 |
PdgX R | GATGAGCAAGTTGGAGTGAATG | ||
PdgX Probe | 6FAM-TGATCGTGTTCTCATTACCGGGCG-QSY | ||
luxS | LuxS F | GAAGTGTCTGAGGCTTGGTTAG | 103 |
LuxS R | CCGTATAGCTTCCGCATTGATAG | ||
LuxS Probe | 6FAM-AGGTGTACAAGATCAAGCTTCTATTCCCG-QSY | ||
dps | Dps F | GGGCTACCACTGGAACATTA | 104 |
Dps R | GTTCAGCCACCTCATCTACTC | ||
Dps Probe | 6FAM-TGCGTGTAATGCAAAGAAGTTTACGCC-QSY | ||
hktE | HktE F | CAACAACCAGACTTTGCTGAAC | 104 |
HktE R | ACGAGGTTGGCTGAAGTAATC | ||
HktE Probe | 6FAM-TCGTATCAACGGAGATGCAGCACA-3IABkFQ | ||
omp26 | Omp26 F | ACCGCACTTGCTTTAGGTATT | 104 |
Omp26 R | GCGATCTGGGTGATGTTGAA | ||
Omp26 Probe | JOE-TTGCTTCAGGCTATGCTTCCGCT-BHQ |